Avian reovirus triggers autophagy in primary chicken fibroblast cells and Vero cells to promote virus production
Identifieur interne : 001422 ( Main/Exploration ); précédent : 001421; suivant : 001423Avian reovirus triggers autophagy in primary chicken fibroblast cells and Vero cells to promote virus production
Auteurs : Songshu Meng [République populaire de Chine] ; Ke Jiang [République populaire de Chine] ; Xiaorong Zhang [République populaire de Chine] ; Miao Zhang [République populaire de Chine] ; Zhizhi Zhou [République populaire de Chine] ; Maozhi Hu [République populaire de Chine] ; Rui Yang [République populaire de Chine] ; Chenli Sun [République populaire de Chine] ; Yantao Wu [République populaire de Chine]Source :
- Archives of Virology [ 0304-8608 ] ; 2012-04-01.
Abstract
Abstract: Avian reovirus (ARV) is an important cause of disease in poultry. Although ARV is known to induce apoptosis in infected cells, the interaction between ARV and its target cells requires further elucidation. In this report, we show that the ARV isolate strain GX/2010/1 induces autophagy in both Vero and primary chicken embryonic fibroblast (CEF) cells based on the appearance of an increased number of double-membrane vesicles, the presence of GFP-microtubule-associated protein 1 light chain 3 (GFP-LC3) dot formation, and the elevated production of LC3II. We further demonstrate that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction by ARV infection. Moreover, treatment of ARV-infected cells with the autophagy inducer rapamycin increased viral yields, while inhibition of the autophagosomal pathway using chloroquine led to a decrease in virus production. Altogether, our studies strongly suggest that autophagy may play a critical role in determining viral yield during ARV infection.
Url:
DOI: 10.1007/s00705-012-1226-x
Affiliations:
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Although ARV is known to induce apoptosis in infected cells, the interaction between ARV and its target cells requires further elucidation. In this report, we show that the ARV isolate strain GX/2010/1 induces autophagy in both Vero and primary chicken embryonic fibroblast (CEF) cells based on the appearance of an increased number of double-membrane vesicles, the presence of GFP-microtubule-associated protein 1 light chain 3 (GFP-LC3) dot formation, and the elevated production of LC3II. We further demonstrate that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction by ARV infection. Moreover, treatment of ARV-infected cells with the autophagy inducer rapamycin increased viral yields, while inhibition of the autophagosomal pathway using chloroquine led to a decrease in virus production. Altogether, our studies strongly suggest that autophagy may play a critical role in determining viral yield during ARV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Meng, Songshu" sort="Meng, Songshu" uniqKey="Meng S" first="Songshu" last="Meng">Songshu Meng</name></noRegion><name sortKey="Hu, Maozhi" sort="Hu, Maozhi" uniqKey="Hu M" first="Maozhi" last="Hu">Maozhi Hu</name><name sortKey="Jiang, Ke" sort="Jiang, Ke" uniqKey="Jiang K" first="Ke" last="Jiang">Ke Jiang</name><name sortKey="Meng, Songshu" sort="Meng, Songshu" uniqKey="Meng S" first="Songshu" last="Meng">Songshu Meng</name><name sortKey="Meng, Songshu" sort="Meng, Songshu" uniqKey="Meng S" first="Songshu" last="Meng">Songshu Meng</name><name sortKey="Sun, Chenli" sort="Sun, Chenli" uniqKey="Sun C" first="Chenli" last="Sun">Chenli Sun</name><name sortKey="Wu, Yantao" sort="Wu, Yantao" uniqKey="Wu Y" first="Yantao" last="Wu">Yantao Wu</name><name sortKey="Wu, Yantao" sort="Wu, Yantao" uniqKey="Wu Y" first="Yantao" last="Wu">Yantao Wu</name><name sortKey="Yang, Rui" sort="Yang, Rui" uniqKey="Yang R" first="Rui" last="Yang">Rui Yang</name><name sortKey="Zhang, Miao" sort="Zhang, Miao" uniqKey="Zhang M" first="Miao" last="Zhang">Miao Zhang</name><name sortKey="Zhang, Xiaorong" sort="Zhang, Xiaorong" uniqKey="Zhang X" first="Xiaorong" last="Zhang">Xiaorong Zhang</name><name sortKey="Zhou, Zhizhi" sort="Zhou, Zhizhi" uniqKey="Zhou Z" first="Zhizhi" last="Zhou">Zhizhi Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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